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. 2017 Aug 21;7:8953. doi: 10.1038/s41598-017-09518-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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High-level expression and purification of TRAIL-Trimer fusion protein. (a) 10% SDS-PAGE analysis of TRAIL-Trimer expression from a fed-batch serum-free cell culture in the bioreactor. 10 µL of cell-free conditioned medium from Day 6 to Day 13 were analyzed under non-reducing condition followed by Coomassie Blue staining. (b) Bioassay analysis of TRAIL-Trimer production in conditioned medium from Day 5 to Day 13. (c) SDS-PAGE and western blot analysis of purified TRAIL-Trimer under either non-reducing or reducing conditions. 2 µg of purified protein was analyzed by a 10% SDS-PAGE and stained with Coomassie Blue. 0.2 µg of purified protein was analyzed by western blot using monoclonal antibody against TRAIL-domain and Trimer-domain, respectively.