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. 2017 Aug 21;7:8943. doi: 10.1038/s41598-017-09306-x

Table 1.

The sequences of sgRNAs7, 25.

Name Strand Sequences (5′–3′)
sgRNA-1 (R1) F CACCAGGTGGTCTGACCGGTAGCG
R AAACCGCTACCGGTCAGACCACCT
sgRNA-2 (R2) F CACCGAGCATATCGTTTGTTACGC
R AAACGCGTAACAAACGATATGCTC
sgRNA-3 (R3) F CACCAATGGCTCCGTCCGTATTCC
R AAACGGAATACGGACGGAGCCATT
sgRNA4 (R4) F CACCAACCACTACGCGAGTCGGCA
R AAACTGCCGACTCGCGTAGTGGTT
sgRNA-5 (R5) F CACCGTGAGAGTTATCTGACCGTA
R AAACTACGGTCAGATAACTCTCAC
sgRNA-6 (R6) F CACCAGGCGATGACGAGATCGCGG
R AAACCCGCGATCTCGTCATCGCCT
sgRNA-7 (R7) F CACCAATCCCGCCCATAATCGAGA
R AAACTCTCGATTATGGGCGGGATT
sgRNA-8 (R9) F CACCAGATTGAACCTACAACCTCG
R AAACCGAGGTTGTAGGTTCAATCT
sgRNA-9 (R8) F CACCTGGAGGCGATGACGAGATCG
R AAACCGATCTCGTCATCGCCTCCA
sgRNA-10 (R10) F CACCGGAGGCGATGACGAGATCGC
R AAACGCGATCTCGTCATCGCCTCC
sgRNA-11 (R11) F CACCGGCAAGTCTGCCATACTAAC
R AAACGTTAGTATGGCAGACTTGCC
sgRNA-12 (R12) F CACCCTAAGTAAGGGATACCGACT
R AAACAGTCGGTATCCCTTACTTAG
sgRNA-13 (R13) F CACCAAGTGGGGAATCCAATATCG
R AAACCGATATTGGATTCCCCACTT
sgRNA-14 (R14) F CACCGAATCCGACTAGGTACCGTG
R AAACCACGGTACCTAGTCGGATTC