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. 2017 Aug 21;7:8328. doi: 10.1038/s41598-017-08270-w

Figure 1.

Figure 1

Diagram of the Selfie digital PCR method (Selfie-dPCR) to analyze gene transcription in relation to the self-encoding gene. Selfie-dPCR measures the amount of DNA and cDNA directly in a cell or tissue lysate that contains native proportions of nucleic acids. It includes four steps: 1) sample and gene-specific primer pre-annealing in duplicate, 2) reverse transcription with enzyme in one duplicate (RT+) and without enzyme (RT) in the other duplicate, 3) digital PCR and 4) nucleic acid quantification. This method allows for the measurement of an RNA transcript in relation to the gene encoding the transcript, thus yielding the absolute number of transcripts/gene at the time of cell or tissue lysis. Diagram drawn by Iris Joval.