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. 2017 Aug 21;7:8328. doi: 10.1038/s41598-017-08270-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Absolute measurement of gene transcription in different tissues with Selfie-dPCR. The numbers of transcripts per gene were measured for single-copy and multiple-copy nuclear genes and in mitochondrial DNA in different tissues. (A) Quantitative analysis of the numbers of transcripts per gene of the single-copy nuclear gene GSK3β in different tissues. Left: representative scatter plot of dPCR in a sample from heart tissue. In heart tissue, there were no significant differences between the RT⁺ and RT⁻ reactions, thus illustrating the lack of detectable Gsk3β transcription. (B) Quantitative analysis of the numbers of transcripts per gene of the multiple-copy nuclear gene RNA18S in different tissues. Left: representative scatter plot of dPCR in a sample from heart tissue. (C) Graph showing the numbers of copies of mtDNA per genome in different tissues. Left: representative scatter plot of dPCR in a heart sample showing amplification of the mtDNA-67 to calculate mtDNA copy number in the left panel and Gsk3β-81 to calculate the number of genome copies in the right panel. (D) Strand-specific analysis of transcription of mitochondrial Heavy (H) and Light (L) strands measured separately and simultaneously in brain (B), heart (H), liver (L) and skeletal muscle (SM). *Significantly different, p < 0.01.