Fig. 1.

Platelets facilitate metastasis in vivo and reduce anoikis. a Plot showing percent of SYTOX Red positive EPCAM + MDAH-2774 tumor cells in ascites after treatment with control IgG or anti-platelet antibody (APA, n = 5, two-sided Student’s t-test). b, c Number of liver nodules b and representative bioluminescence imaging pictures c 5 weeks after intrasplenic injection of 2 × 10⁶ SW620 colon cancer cells (n = 10, two-sided Student’s t-test). d Number of dead (SYTOX Red positive, black) and living (SYTOX Red negative, red) HEYA8 cells after 72 hours of low attachment and/or co-incubation with increasing numbers of platelets (n = 3, two-sided Student’s t-test). e Number of dead (SYTOX\ Red positive, black) and living (SYTOX Red negative, red) OVCAR8, SKOV3, MDAH-2774 and SW620 cells after 72 h of low attachment and/or co-incubation with 100 × 10⁶ platelets (n = 3, two-sided Student’s t-test). f Percentage of HEYA8 or OVCAR8 human ovarian cancer cells in sub-G1 phase of the cell cycle after 72 h under low-attachment conditions (n = 3, two-sided Student’s t-test). g Protein analysis and quantification of full length and cleaved PARP in HEYA8 and OVCAR8 cells after 72 h under low-attachment conditions (short exposure of PARP upper panel, long exposure of PARP lower panel, n = 3, two-sided Student’s t-test). GAPDH was used as a loading control. Bars and error bars represent mean values and the corresponding SEMs (*p < 0.05, **p < 0.01, ***p < 0.001)