Fig. 2.

Platelets induce a YAP1-specific gene signature in cancer cells. a Heat map showing differentially regulated proteins as analyzed by reverse phase protein array (RPPA) in HEYA8 and HEYA8 co-incubated with platelets for two hours (n = 2). b Top 10 down- and upregulated proteins (+plts vs. control) after RPPA analysis. c Heat map depicting differentially regulated genes in HEYA8 or HEYA8 cells co-incubated with 100 × 10⁶ platelets under low-attachment conditions for 24 hours (n = 3, p < 0.001). d Enriched pathways with indicated p-values of upregulated genes in platelet co-incubated HEYA8 cells using Gene Set Enrichment Analysis (GSEA, www.broadinstitute.org/gsea). e Upstream analysis of gene expression changes in HEYA8 ovarian cancer cells co-incubated with platelets using Ingenuity Pathway Analysis (IPA, http://www.ingenuity.com/products/ipa). f Random walk analysis highlighting YAP1 protein as the main connector to transcriptional changes in HEYA8 co-incubated with platelets. g QRT–PCR analysis of YAP1, NDRG1, DDIT4, CDC20, CTGF, MCM5, MCM6, and MCM10 in HEYA8 cells after 24 and 48 h of co-incubation with platelets. 18S was used as the housekeeping gene (n = 3, two-sided Student’s t-test). h QRT–PCR analysis of YAP1, NDRG1, DDIT4, CDC20, CTGF, MCM5, MCM6, and MCM10 after YAP1 knockdown with two different siRNAs (72 hours after transfection, n = 3). 18S was used as the housekeeping gene (n = 3, two-sided Student’s t-test). Bars and error bars represent mean values and the corresponding SEMs (*p < 0.05, **p < 0.01, ***p < 0.001)