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. 2017 Aug 21;7:8959. doi: 10.1038/s41598-017-08870-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CoMot and CoMot+ cells in a 3OC6HSL gradient show directional movement towards the 3OC6HSL source: A 3OC6HSL gradient was established by adding 0.02 μmoles of 3OC6HSL to a Whatmann membrane and allowing it to diffuse for 8 h. 1 μM of 3OC6HSL would be the final concentration if 0.02 μmoles of 3OC6HSL diffused uniformly through the plate. CoMot, CoMot+ or cells that constitutively express motA (∆motA transformed with a plasmid containing P_esaR-motA) were then inoculated at the centre of the plate. Images were obtained following 0, 18, 24 and 36 h of incubation at 30 °C. The assay was run in triplicate for each strain and representative images are shown.