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. 2017 Aug 21;7:8952. doi: 10.1038/s41598-017-08357-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The loss of Arpc2 leads to impaired T cell homeostasis. (a) Survival was assessed in naïve T cells using flow cytometry analysis with Annexin V and DAPI staining. (b) The percentage statistics for Annexin V of naïve T cells obtained from Ctrl and Arpc2-TKO mice are illustrated. (n = 3). (c) Naïve CD4⁺ or CD8⁺ T cells isolated from CD45.2⁺ Arpc2-TKO mixed equally with CD45.1⁺ congenic Ctrl mice were labeled with carboxyfluorescein succinimidyl ester (CFSE) and co-transferred into Rag1 ^−/− mice. After 7 days the mice were sacrificed, and the lymphocytes were analyzed. (d) The percentage statistics for CFSE dilution proliferated CD4⁺ and CD8⁺ T cells was analyzed (n = 3). (e-h) A mixture of equal number of CD45.1⁺ Ctrl and CD45.2⁺ Arpc2-TKO bone marrow cells was transferred into Rag1 ^−/− mice. Flow cytometry analysis of CD4 and CD8 expression of T cells in (e) lymph nodes and (g) spleen. The percentage statistics for CD4⁺ and CD8⁺ T cells in (f) lymph nodes (n = 4) and (h) spleen (n = 6). The data are means ± S.D., for all panels: *P < 0.01; ***P < 0.001; ****P < 0.0001 by Student’s t-test, N.S.: no significance. All results are representative from at least three independent experiments.