Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 21;7:8952. doi: 10.1038/s41598-017-08357-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Peripheral Arpc2-TKO T cells show impaired surface TCR maintenance and immune synapse formation. (a) Representative histograms and (b) Mean fluorescence intensity (MFI) of surface (n = 7) and total (n = 5) TCRβ levels in peripheral T cells obtained from Ctrl and Arpc2-TKO mice. (c) MFI of surface TCRβ levels of control and Arpc2 knockdown peripheral T cells (n = 5) (d) T cells isolated from the lymph nodes of Ctrl and Arpc2-TKO mice were incubated with anti-CD3 and anti-CD28 activator beads for 15 min and stained with Phalloidin-Tritc and primary antibodies against Arpc2 and CD3ζ. They were then incubated with the appropriate secondary antibodies and imaged using microscopy. Bar is 5 μm. Representative images are shown. (e) The histogram show the percentage of conjugates harboring CD3ζ and F-actin at the immune synapse. At least 30 cells were analyzed for each experiment. (experiment, n = 5). (f) Lymphocytic T cells were labeled with CFSE and they were unstimulated or stimulated with anti-CD3 and anti-CD28 activator beads at a 2:1 (cell: bead) ratio for 48 h. The data are means ± S.D., for all panels: *P < 0.05; **P < 0.01; ***P < 0.001 by Student’s t-test, N.S.: no significance. All results are representative from at least three independent experiments.