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. 2017 Jul 4;292(33):13541–13550. doi: 10.1074/jbc.M116.772426

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — Lys-776, His-917, and His-936 on p110α are critical regulators of PI3Kα lipid kinase activity. A, SDS-PAGE analysis of PI3Kα constructs purified by anion-exchange chromatography. WT and eight mutants are shown. B, gel-exclusion chromatograms of the wild-type enzyme (shown in blue) and one of the mutants-H917A-p110α/p85α (shown in orange) as an example to illustrate the preservation of the oligomeric state of the mutants. Molecular weight standards (Bio-Rad) are shown in green, and peaks A, B, C, D, and E represent 678 kDa, 158 kDa, 44 kDa, 17 kDa, and 1.35 kDa, respectively. mAU, milliabsorbance units. C and D, comparison of lipid kinase activity of WT and mutants. Michaelis-Menten plots are shown with ATP (C) and PIP₂ (D). Activity (V) is expressed in pmol/min. Error bars represent S.D.