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. 2017 Jun 21;292(33):13551–13564. doi: 10.1074/jbc.M117.780973

Figure 3.

Figure 3.

HuR depletion decreases ERBB2 mRNA stability and translation. A and B, the levels of HuR protein in MCF7 and T47D control and TAMR cells were determined by Western blotting, with β-actin as input control. C, MCF7/TAMR cells transfected with either HuR siRNA or control siRNA were treated with actinomycin D (10 μg/ml) 48 h after transfection and harvested at 0, 2, 4, 6, and 8 h for RNA extraction and reverse transcription. ERBB2 mRNA levels at the different time points were measured by qPCR, using GAPDH as input control. D, MCF7/TAMR cells transfected with either HuR siRNA or control siRNA were harvested after 48 h, and ERBB2 mRNA levels were measured with RT-qPCR, using GAPDH as input control. E, luciferase reporter activities were determined in MCF7/TAMR cells co-transfected with either control siRNA or HuR siRNA and either psi-CHECK2-Vec or psi-CHECK2-ERBB2-3′-UTR, as indicated. F, ERBB2 and HuR protein levels in MCF7/TAMR and T47D/TAMR cells transfected with either the scrambled siRNA (siNC) or HuR siRNA were analyzed by Western blotting, with β-actin as input control. *, p < 0.05. Error bars, S.E.