Figure 8.

FH2/actin interaction is regulated through the DID/DAD-CT interaction. A, representative polymerization kinetics of actin in the absence and presence of DID, FH1–FH2, and cDAAM, as indicated. [actin] = 2 μm (containing 5% pyrenyl actin), [FH1–FH2] = 5 μm, [cDAAM] = 0.51 μm. B, relative polymerization rate of cDAAM catalyzed actin assembly as a function of DID concentration. Dashed line corresponds to the fit of the data using Equation 1. The fit gave half-inhibition of cDAAM by DID at 30.5 ± 14.31 nm. a.u., arbitrary units. The relative rate of spontaneous actin assembly is indicated by gray dashed line.