Figure 1.

Nitration of human hemopexin occurs in vivo at Tyr-199. Nitration of human hemopexin occurs in vivo at Tyr-199 detected by LC-MS/MS analysis. SEQUEST was used to identify Tyrⁿ-199 on the tryptic peptide YYⁿCFQGNQFLR (see Table 1 for XCorr value). Matched b and y ions are shown in bold in the ion diagram (above) and are indicated next to corresponding peaks in the MS/MS spectrum (below). Similar high quality spectra were obtained from rabbit and rat hemopexin (see supplemental Figs. S1 and S2).