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. 2017 Jun 28;292(33):13702–13713. doi: 10.1074/jbc.M117.797332

Figure 2.

Figure 2.

Quantitative lipid analysis of HIV-1–producing cells and purified HIV-1 particles. A and B, lipid composition of OptiPrep-purified HIV-1NL43 WT or ΔNef particles produced in the absence or presence of SERINC5 (A) and of the corresponding 293T HIV-1–producing cells (B). Lipid classes are standardized to all lipids measured. Cer, ceramide; Chol, cholesterol; -O, ether or odd-numbered fatty acyl residue. Data are presented as mean ± S.D. of three independent experiments, except for ΔNef particles produced in the presence of SERINC5 (n = 2). Note that low-abundant lipid classes are presented in separate graphs on the right. C, relative contribution of SP, GP, and sterols (ST, cholesterol) to the lipid composition of HIV-1–producing cells and OptiPrep-purified HIV-1NL43 WT or ΔNef particles produced in the absence or presence of SERINC5.