Figure 7.

Smad1 and Smad5 mediate the BMP9- and BMP10-induced up-regulation of E-selectin, VCAM-1, and ICAM-1 in TNFα-stimulated HAECs. HAECs were siRNA transfected, then treated with BMP9 or BMP10 (5 ng/ml, 16 h) prior to TNFα treatment (0.05 ng/ml, 4 h). Surface expression of E-selectin (FITC-conjugated anti-human E-selectin) (A), VCAM-1 (PE-Cy5-conjugated anti-human VCAM-1) (B), and ICAM-1 (APC-conjugated anti-human ICAM-1) (C) was assessed using flow cytometry. Forward scatter and side scatter gating was applied to the HAEC population. Data are shown as median fluorescence intensity (MFI) expressed as fold-change relative to HAECs transfected with siRNA control pool (siCP). Experiments were performed in triplicate and the data are representative of n = 3 biological repeats. Error bars represent mean ± S.E. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.