Figure 1.

Viperin interacts with the late-acting CIA-targeting proteins. HEK293T cells were transiently transfected with plasmids encoding wild-type viperin, FLAG-tagged CIA1 (A), HA-tagged CIA2B (B), FLAG-tagged MMS19 (C), and Myc-tagged CIA2A (D) as indicated (+). After growth for 1 day, cell extracts were subjected to anti-viperin IP. Whole-cell lysates (input) and immunoprecipitated proteins were analyzed by immunoblotting using antibodies as indicated. E, FLAG–viperin expression in FLP-IN T Rex cells was induced by tetracycline (+ Tet, 1 μg/ml in culture medium) for 24 h, followed by IP with an anti-FLAG antibody and sample analysis by immunoblotting. Tubulin served as loading control. The blots are representative of three independent experiments.