Figure 4.

CIA-targeting factors CIA1, CIA2B, and CIA2A directly interact with viperin. A, HEK293T cells were transiently transfected with control siRNAs (CsiR) or siRNAs directed against the indicated CIA-targeting factors. In addition, cells received plasmids coding for the remaining CIA-targeting complex components and FLAG–viperin as indicated (+). Anti-FLAG immunoprecipitation and sample analysis were performed according to Fig. 2. Tubulin and FLAG staining served as reference, and representative blots are shown. The chemiluminescence associated with CIA1 (B), MMS19 (C), CIA2B (D), and CIA2A-myc (E) was quantified as in Fig. 3. Mean values and standard deviation from three independent experiments are shown.