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. 2017 Jun 27;292(33):13890–13901. doi: 10.1074/jbc.M117.783860

Figure 2.

Figure 2.

Knockdown of SLC13A5 inhibits cell cycle progression in HepG2 and Huh7 cells. A and B, cell cycle analysis of HepG2 and Huh7 cells 6 days after infection with sh13A5 or shCon. The percentage of cells in different cell cycle phases was compared between cells infected with sh13A5 and shCon. C, RT-PCR was used to measure the mRNA expression of cyclin B1. D, Western blotting was carried out to measure the protein levels of cyclin B1 in HepG2 and Huh7 cells infected with sh13A5 or shCon. E and F, expression of p21 mRNA and protein was measured in HepG2 and Huh7 cells infected with sh13A5 or shCon. Data were collected from three independent experiments and expressed as mean ± S.D. *, p < 0.05; **, p < 0.01.