Figure 3.

SLC13A5 knockdown does not affect apoptosis of HepG2 and Huh7 cells. A and B, HepG2 and Huh7 cells were infected with sh13A5 or shCon for 6 days or treated with MG132 (1 μm) for 24 h as positive control. Cell density was visualized under phase-contrast microscopy. Representative fluorescence photographs depict apoptotic nuclei (white arrows) and PI-positive secondary necrotic nuclei (yellow arrows). C, caspase 3 activity was analyzed with Western blotting to detect the large fragment (17/19 kDa) of cleaved caspase 3 in HepG2 and Huh7 cells with or without SLC13A5 knockdown. β-Actin was used to normalize protein loading. D, DNA fragmentation was illustrated on agarose electrophoresis of genomic DNA extracted from HepG2 and Huh7 cells infected with sh13A5 or shCon or treated with MG132 (2 μm) for 48 h. Data presented are representative images from three independent experiments.