Figure 6.

SLC13A5 depletion inhibits growth of HepG2 xenograft in vivo. HepG2 cells were infected with lentivirus encoding sh13A5 or shCon. Four days after culture, equal numbers of HepG2-sh13A5 and HepG2-shCon cells were injected into the nude mice for xenograft formation as detailed under “Experimental Procedures.” A–C, tumor growth curves (A), images (B), and weights (C) for xenografts formed between sh13A5-1 (KD) and shCon (control) groups were measured during or at the end of 6 weeks. D–F, the mRNA expression of SLC13A5 (D), cyclin B1 (E), and ACLY (F) was measured by RT-PCR. G, Western blotting was used to measure the protein expression of SLC13A5, phospho-AMPKα (p-AMPKα), total AMPKα, phospho-mTOR (p-mTOR), total mTOR, ACLY, cyclin B1, and β-actin. Data are expressed as mean ± S.D. *, p < 0.05; **, p < 0.01.