Figure 8.

NEIL1 interferes with TDG activity on a T/G mismatch: 50 nM TDG and varying amounts of inactive NEIL1 P2T or NEIL1 wt were incubated (37 °C, 60 min) with 20 nM dsDNA containing a radiolabelled strand with T/G mismatch. DNA was then broken at AP-sites by in alkaline conditions and analysed by denaturing gel electrophoresis and autoradiography. Interference of NEIL1 with TDG glycosylase activity towards the T/G mismatch substrate was independent of the NEIL1 catalytic activities. The NEIL1 inhibits both full-length TDG (A) and TDGcd (B).