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NEIL1 glycosylase activity is not affected by TDGcd: 100 nM NEIL1 and increasing amounts of the catalytically impaired TDGcd N140A were incubated (37 °C, 60 min) with 20 nM dsDNA containing a radiolabelled strand containing either a 5caC or 5OHC base opposite G. After the enzymatic reaction, reaction products were subjected to alkaline conditions to break DNA strands at AP-sites.
