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. 2017 Aug 21;7:8990. doi: 10.1038/s41598-017-08996-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GPR61, GPR62 and GPR135 expression on cAMP and inositol phosphate production in HEK293T cells. Basal (A–C) and forskolin-stimulated (D–F) cAMP accumulation in HEK293 cells as a function of transfected vector DNA expressing HA-GPR61, HA-GPR62 and HA-GPR135. Inositol phosphate (IP1) production was determined in HEK293T cells transfected with different quantities of HA-GPR61 (G), HA-GPR62 (H) and HA-GPR135 (I) expression vectors in the absence or presence of 1 µM melatonin (MLT). (J) Representative western blot of HA-GPR61, HA-GPR62 or HA-GPR135 expression control of experiments shown in panels A–I. Data are expressed as mean ± SEM. from three independent experiments performed in triplicates. ***P < 0.0001.