Table 2.
CDR3 sequences of selected nanobodies.
| Name | CDR3 sequence | length (aa) | library (Fig. 2) |
|---|---|---|---|
| VHH-1 | A D K W G G Y C G G V A D I A H F G Y | (19) | 54A1 |
| VHH-5 | S T R V W G G Y C G G L D D A T N N D | (19) | 54A1/DBO2 |
| VHH-A6 | T E T W G P Y C G: Q A S P A Y F P Y | (18) | DBO2 |
| VHH-9 | D V Y R G G Y C S A Y A G R:: F S Y | (17) | 54A2 |
| VHH-28 | D V N R G G F C Y I E D W Y:: F S Y | (17) | 54A2 |
| VHH-33 | D D L R G G Y C S S S P T L:: Y T Y | (17) | 54A1 |
| VHH-23 | G T R P S I F C Y K G D R W:: Y S A | (17) | 54A2 |
| VHH-43 | S Y Q F F S S E P S L S A R R: Y T D | (18) | 54A1 |
Sequences were aligned by eye. The conserved cysteine forming a disulfide with a cysteine in CDR1 is underlined; “:” indicates deleted amino acids.