Table 2.
Plasmids used in this study.
| Plasmid | Characteristics | Source or reference |
|---|---|---|
| pBAD24 | Arabinose inducible expression vector | Guzman et al., 1995 |
| pBAD::relCg | pBAD24 carrying the gene rel (cg1861) from C. glutamicum CR099 | This study |
| pBAD::relP*Cg | pBAD24 carrying the gene relP* (cg1330) from C. glutamicum CR099 | This study |
| pBAD::relPCg | pBAD24 carrying the gene relP from C. glutamicum AS1.542 | This study |
| pBAD::relSCg | pBAD24 carrying the gene relS (cg2324) from C. glutamicum CR099 | This study |
| pK18mobsacB | Suicide vector for gene deletion by homologous recombination | Schäfer et al., 1994 |
| pK18mobsacB_relCg | pK18mobsacB carrying the up- and downstream region (500 bp respectively) of the gene rel from C. glutamicum CR099 | This study |
| pK18mobsacB_relP*Cg | pK18mobsacB carrying the up- and downstream region (500 bp respectively) of the gene relP* from C. glutamicum CR099 | This study |
| pK18mobsacB_relSCg | pK18mobsacB carrying the up- and downstream region (500 bp respectively) of the gene relS from C. glutamicum CR099 | This study |
| pRedET | Red/ET expression plasmid | Gene Bridges |
| 708-FLPe | FLPe expression plasmid | Gene Bridges |
| pTXB1 | E. coli expression vector, carrying the self-cleavable Mxe intein/chitin binding domain | New England Biolabs |
| pTXB1::relP*Cg | pTXB1 carrying the gene relP* (cg1330) from C. glutamicum CR099 | This study |
| pTXB1::relSCg | pTXB1 carrying the gene relS (cg2324) from C. glutamicum CR099 | This study |
| pTXB1::relCg | pTXB1 carrying the gene rel (cg1861) from C. glutamicum CR099 | This study |