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. 2017 Aug 21;8:1423. doi: 10.3389/fpls.2017.01423

FIGURE 1.

FIGURE 1

Molecular analysis of three transgenic chickpea lines. (A) PCR analysis of transgenic chickpea progenies. Lanes are as follow, L1: 1 kb DNA Ladder (Thermo Scientific, United States); IPCa1 (Lanes 1–6): progenies from IPCa1; IPCa2 (Lanes 7–11): progenies from IPCa2; IPCa3 (Lanes 12–16): progenies from IPCa3; N: no template control; WT: wild-type (non-transgenic line); P: positive control (Plasmid); L2: 1 kb DNA ladder (Thermo Scientific, United States). (B) Southern blot analysis of three events using cry1Aabc specific probe in T4 generation. Copy number (CN) identification using HindIII. Cassette release (CR) (Cry1Aabc gene) in transgenic lines using HindIII and EcoRI. Lanes are as follow, L: DIG labeled mol. wt. marker (Roche Diagnostics GmbH, Mannheim, Germany); A: IPCa1 event; B: IPCa2 event; C: IPCa3 event; D: negative control (non-transgenic DCP92-3); E: positive control. (C) Western blot analysis (T4 Generation) [Lanes 1–3: IPCa2 progenies; Lanes 4–5: IPCa3 progenies; and Lanes 6–7: IPCa1 progenies; NC: negative control; PC: positive control (Purified Cry)].