Figure 6.

Functional interaction network of protein kinases and phosphatases required for normal protein degradation in muscle suggest that protein phosphatase 2A is a central node. (A) Kinases and phosphatases that were identified as required for lack of pathological protein degradation in muscle were examined for functional interactions in WormBase,22 GeneMANIA,26 and PhosphoPOINT.27 Kinases are indicated in blue and phosphatases in yellow. (B) Western blot analysis of MEK activation in response to knockdown of phosphatases identified in network analysis and as triggering autophagy. Quantification of MEK phosphorylation from three separate RNA interference experiments is displayed above representative blots. *P < 0.05, t‐test (graph pad prism).