Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 23;2(4):e00274-17. doi: 10.1128/mSphere.00274-17

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Wiedman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 5 — One hundred picomoles of aptamer (Rd 13, Rd 9, or the library) was titrated into 100 pmol of various BODIPY-labeled drugs (posaconazole [Pos], isavuconazole [ISV], and caspofungin [CSF]) (A). Titration of Rd 13 into PosBD caused a significant change in anisotropy. Titration of Rd 13 into other BODIPY-labeled drugs or Rd 9/library into PosBD caused little changes in anisotropy. These data suggested that Rd 13 binds best to posaconazole. Aptamers of different lengths were titrated into 100 pmol of PosBD (B). The full-length Rd 13 aptamer caused the greatest change in anisotropy, and the truncated and scrambled versions showed decreased amounts of anisotropy change. The scrambled version only caused a change proportional to that caused by a chelator, EDTA.