FIG 5 .

A ΔfepB mutant resembles a triple siderophore mutant in vivo. Mice were inoculated i.n. with 2 × 10⁴ CFU of the WT (KPPR1S; black circles) or the ΔfepB (VK412; open squares, small closed circles), ΔentB mutant (VK087; open diamonds), or ΔentBybtS (VK089; black squares) mutant. At 24 or 72 hpi, mice were sacrificed and their lungs and spleens were homogenized and plated for bacterial enumeration. Each symbol represents one mouse. The dotted line indicates the limit of detection, and symbols on the dotted line indicate that CFU counts were below the limit of detection. The data are from an individual representative experiment. Mann-Whitney tests were performed for statistical analysis. *, P < 0.05.