TABLE 2 .
Bacterial strains and plasmids used in this work
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoP recA1 endA1 hsdR17(rK− mK−) | Invitrogen |
| S17-1 λpir | Tpr Strr recA thi pro hsdR hsdM+ RP4-2-Tc::Mu Km Tn7 λpir (lysogen) | 72 |
| K. pneumoniae | ||
| KPPR1 | Rifr derivative of ATCC 43816 | 34 |
| KPPR1S | Strr derivative of KPPR1 | This work |
| VK060 | KPPR1 cpsB::Tn5Kn2 | 34 |
| VK082 | smr mutant | This work |
| VK087 | KPPR1 ΔentB | 33 |
| VK088 | KPPR1 ΔybtS | 33 |
| VK089 | KPPR1 ΔentB ΔybtS | 33 |
| VK131 | KPPR1 ΔromA | This work |
| VK174 | KPPR1S ΔramA | This work |
| VK266 | KPPR1S Δorf82 ΔramA ΔromA | This work |
| VK269 | KPPR1S Δrnd | This work |
| VK270 | KPPR1S Δorf82 | This work |
| VK274 | KPPR1S Δsmr_A | This work |
| VK275 | KPPR1S Δsmr_B | This work |
| VK276 | KPPR1S Δsmr_C | This work |
| VK320 | KPPR1S Δfes | This work |
| VK321 | KPPR1S ΔybdB2 entABEC (Δentsyn) | This work |
| VK411 | KPPR1S ΔentS | This work |
| VK412 | KPPR1S ΔfepB | This work |
| VK413 | KPPR1S fepD::pKAS46 | This work |
| VK555 | KPPR1S ΔfepB2 | This work |
| Plasmids | ||
| pKAS46 vector | Kanamycin resistance, suicide vector, rpsL+ | 70 |
| pK03 vector | sacB, temperature-sensitive origin of replication | 73 |
| pPROBE vector | Kmr, gfp expression vector | 77 |
| pKO3ΔromA | romA flanking region in pKO3 | This work |
| pKO3ΔramKO | smr flanking region in pKO3 | This work |
| pKAS46ΔramA | ramA flanking region in pKAS46 | This work |
| pKAS46Δorf82 | orf82 flanking region in pKAS46 | This work |
| pKAS46Δorf82ramAromA | orf82 ramA romA flanking region in pKAS46 | This work |
| pKAS46Δrnd | rnd flanking region in pKAS46 | This work |
| pKAS46ΔfepB | fepB flanking region in pKAS46 | This work |
| pKAS46Δsmr_A | smr_A flanking region in pKAS46 | This work |
| pKAS46Δsmr_B | smr_B flanking region in pKAS46 | This work |
| pKAS46Δsmr_C | smr_C flanking region in pKAS46 | This work |
| pKAS46Δfes | fes flanking region in pKAS46 | This work |
| pKAS46ΔentS | entS flanking region in pKAS46 | This work |
| pKAS46ΔybdB2entABEC | ybdB2 entABEC (Δentsyn) flanking region in pKAS46 | This work |
| pfepD::pKAS46 | Disruption of fepD | This work |
| pY4 | ybtA promoter in pPROBE | 33 |