TABLE 3 .
Primers used in this study
| Primer | Sequencea (5′ to 3′) | Description |
|---|---|---|
| MP66 | TGACTAGATATCGCTGATTACCGAAGCGGACTG | 5′ flank forward ΔramA |
| MP67 | TGCATATCTAGAGGAAATCGTCATATGCTCTCT | 5′ flank reverse ΔramA |
| MP68 | TGCATATCTAGACACTGAGGCGCGCCTCTCCTG | 3′ flank forward ΔramA |
| MP69 | TCGATAGCGGCCGCCGACTGGCGCTGTACATCGCG | 3′ flank reverse ΔramA |
| MP114 | TGACTAGATATCTCGCCCGAGGGCGTCGTAAAC | 5′ flank forward Δorf82 |
| MP71 | TGCATATCTAGACTCGAGCGGTAAACCAGGAGA | 5′ flank reverse Δorf82 |
| MP72 | TGCATATCTAGACAGTGGATGTTTCATGTCATG | 3′ flank forward Δorf82 |
| MP115 | TCGATAGCGGCCGCGGGATGAACCGTATCAACGGC | 3′ flank reverse Δorf82 |
| MP124 | TGACTAGATATCCGATTTTGCCTGCTATGCGCA | 5′ flank forward Δrnd |
| MP125 | TGCATATCTAGACATCGGCGGGGGTAAGCGCGG | 5′ flank reverse Δrnd |
| MP126 | TGCATATCTAGAGTTCACCCGGTCGCCCAGCGG | 3′ flank forward Δrnd |
| MP127 | TCGATAGCGGCCGCGCCACGGCAGGTCTGGCAGCA | 3′ flank reverse Δrnd |
| MP103 | TGACTAGATATCGGCGTCGTAAACTTTGGGTTA | 5′ for Δorf82 ramA romA |
| MP104 | TGCATATCTAGATTCCAGTGGATGTTTCATGTC | 5′ rev Δorf82 ramA romA |
| MP105 | TGCATATCTAGACTGACCAGACAAAAGCCCCCA | 3′ for Δorf82 ramA romA |
| MP106 | TCGATAGCGGCCGCCGACAGCTGGCACATTTCGTT | 3′ rev Δorf82 ramA romA |
| MP171 | TCGATAGCGGCCGCCTGTGCGCTCCCTGCGCCATG | 5′ flank forward smrΔA |
| MP172 | TGCATATCTAGACTGGCGAAGTAGGGGAGGGGG | 5′ flank reverse smrΔA |
| MP173 | TGCATATCTAGAACCGAGATTTAATCTCTCCAC | 3′ flank forward smrΔA |
| MP174 | TGACTAGATATCTCCAACTTTTGGGGTGCAGTC | 3′ flank reverse smrΔA |
| MP175 | TGACTAGATATCCCATGCGCTTGCGCGGGCCTA | 5′ flank forward smrΔB |
| MP176 | TGCATATCTAGAGCTTACGATATTTCCAATCCG | 5′ flank reverse smrΔB |
| MP177 | TGCATATCTAGATGCGCCTCATTAAGCGGGTCC | 3′ flank forward smrΔB |
| MP178 | TCGATAGCGGCCGCAATGACAGAATGTTAAGGACA | 3′ flank reverse smrΔB |
| MP179 | TGACTAGATATCTGCGCCTCATTAAGCGGGTCC | 5′ flank forward smrΔC |
| MP180 | TGCATATCTAGAAGTCACGCTATACATAGGGTT | 5′ flank reverse smrΔC |
| MP181 | TGCATATCTAGAGCGCACCCTGGCGGAGCCACT | 3′ flank forward smrΔC |
| MP182 | TCGATAGCGGCCGCATTAACGACAGGTTGCGCGAA | 3′ flank reverse smrΔC |
| MP282 | TGACTAGATATCAGAATTTAACAACACCGAAAC | 5′ flank forward ΔybdB2 entABEC |
| MP192 | TGCATATCTAGAACCGCGGTGCTGGGCTAAGAA | 5′ flank reverse ΔybdB2 entABEC |
| MP193 | TGCATATCTAGAAGCCAGTGACGTTTCCATATC | 3′ flank forward ΔybdB2 entABEC |
| MP194 | TCGATAGCGGCCGCGCAACCTCGCTCCACTGGCGC | 3′ flank reverse ΔybdB2 entABEC |
| MP195 | TGACTAGATATCGGATATAGAGCTCGGAAGGCT | 5′ flank forward ΔfepB |
| MP196 | TGCATATCTAGAGAAGTTCACGTCATCGCATCC | 5′ flank reverse ΔfepB |
| MP197 | TGCATATCTAGACTGTTCGGCTAACGCGGGCTG | 3′ flank forward ΔfepB |
| MP198 | TCGATAGCGGCCGCCGCTGGCGCTTGTCGGCGTGC | 3′ flank reverse ΔfepB |
| MP199 | TGACTAGATATCGCGCTCTGCTGGTGCTCCAGC | 5′ flank forward ΔentS |
| MP200 | TGCATATCTAGAATTGTCAACGAAAGTTAAGTA | 5′ flank reverse ΔentS |
| MP201 | TGCATATCTAGAGGATTGTCGGTTCATTACAGC | 3′ flank forward ΔentS |
| MP202 | TCGATAGCGGCCGCCGGGTGAGCGTCTGCATCAGC | 3′ flank reverse ΔentS |
| MP207 | TGACTAGATATCGCGCGGCAACCAGCGGTAAAC | 5′ flank forward Δfes |
| MP208 | TGCATATCTAGAGGCCAACGCGAACCGATTATT | 5′ flank reverse Δfes |
| MP244 | TGCATATCTAGATGCGCCTCATTAAGCGGGTCC | 3′ flank forward Δfes |
| MP231 | TCGATAGCGGCCGCAATGACAGAATGTTAAGGACA | 3′ flank reverse Δfes |
| MP313 | TGACTAGATATCCCTTAGCCGCCGCGCTTA | 5′ forward fepD::kan |
| MP314 | TGCATATCTAGATTGCGGGTGAGCGTCTGC | 3′ reverse fepD::kan |
| ramKOA5′INsmaI | TCCCCCGGGACCGCTTTGACGGTCAT | 5′ flank forward smr |
| ramKOA3′IN2 | CGCGGTAGATTCCAAACATA | 5′ flank reverse smr |
| ramKOB5′IN | ATCCTGACCAGACAAAAGCCCCATCC | 3′ flank forward smr |
| ramKOB3′INSma | TCCCCCGGGGACAGCTGGCACATTTC | 3′ flank reverse smr |
| romA5′inXba | GCTCTAGAGCCAGTCCGCTTCGGTAA | 5′ flank forward ΔromA |
| romA5′in | CGACTTTCATCGCTTTCCTAATA | 5′ flank reverse ΔromA |
| romA3′in | CGTCATATGCTCTCTCCTCTGAT | 3′ flank forward ΔromA |
| romA3′inXbaI | GCTCTAGAGCACAGCTTAGCCAGGTG | 3′ flank reverse ΔromA |
a
Restriction sites are in bold.