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. 2017 Aug 21;7:8993. doi: 10.1038/s41598-017-08356-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Markerless integration with short homologous arms. (a) Schematic of the transforming donor DNA fragment targeting the Ura3 locus and the CRISPR-Cas9 plasmid targeting two sites (indicated by lightning bolts) flanking the Ura3 CDS. Dashed boxes indicate homologous regions. (b) Homologous recombination rate ((5-FOA resistant * PCR check)/ transformants (G418 resistant) * 100).