Figure 2.

Representative images of stained control and R6/2 NMJs from 12-week-old mice. Motor nerves and terminals were identified by a combination of neurofilament staining (SMI-31) of axons and SV2b staining of synaptic vesicles (both in green; nerve). Motor endplates were labeled by staining of nicotinic acetylcholine receptors with rhodamine-conjugated α-bungarotoxin (red; nAChRs). The regions of superimposed nerve and nAChRs are yellow in the merged images. In both the control and R6/2 fibers, all nAChRs were directly aligned with nerve terminals. Scale bar: bottom right, 30 μm.