The mEPC charge, eEPC charge, and QC in control and R6/2 NMJs in low extracellular Ca2+ (0.5 mm) and high Mg2+ (4 mm). A, Superimposed representative eEPC traces from control and R6/2 NMJs with an inset of superimposed representative mEPC traces from the same NMJs under two-electrode voltage-clamp. B, Histograms of average control (solid black bars) and R6/2 (striped gray bars) mEPC charge, eEPC charge, and QC. Regions of overlap as black striped bars.