Figure 3.
Functional complementation of the Δhbt1 and Δhbt2 mutations. The mutants Δhbt1/Δhbt1 and Δhbt2/Δhbt2 were transformed with the pPK5-derived plasmid constructs carrying either HBT1, HBT2 or HBT3 gene. The transformants were cultivated overnight in SD + MPA medium at 28 °C, washed with water, diluted to concentration 5 × 105 cells/ml and plated in fivefold dilutions onto indicated synthetic media buffered with 100 mM Tris-HCl to pH 7.5. The plates were incubated for 7 days at 28 °C. Transformants with the vector pPK5 were used as a control.