Table 4.
Homozygous diploid deletion strains with random budding pattern
| Category | Gene (ORF) | Budding patterna | Morphological phenotype | Mat a budding pattern | Bud8p localization |
|---|---|---|---|---|---|
| Ribosome proteins | |||||
| Strong phenotype | RPS27B | >50% R | Round 2 | WT | |
| RPL14A | >50% R | WT | WT | ||
| RPS0B | >50% R | Round 2 | WT | ||
| RPL22A | >50% R | Round 3, clumpy 2 | WT | ||
| RPS17A | >50% R | Small 2 | WT | ||
| RPS7A | >50% R | Round 2+ | WT | ||
| RPL7A | >50% R | WT | WT | ||
| RPL39 | >50% R | Round 2, small 2 | NT | ||
| Weak phenotype | RPL12B | <30% R | Small 2 | WT | |
| RPL27A | <40% R | Round 2 | WT | ||
| RPS28B | <50% R | Round 2 | WT | ||
| RPS30A | <30% R | WT | WT | ||
| RPS29A | <30% R | WT | WT | ||
| RPS1B | <50% R | WT | WT | ||
| RPS18B | <30% R | Small 3 | WT | ||
| Vesicular transport proteins | |||||
| Strong phenotype | BST1 | >50% R | Round 4 | WT | WT |
| CLC1 | >50% R | Round 3, clumpy 3 | WT | Weak or ND | |
| YPT31 | >50% | Round 3 | WT | More patch | |
| VMA5 | >50% R | Round 2 | WT | WT | |
| VAM8 | >50% R | Large 2 | WT | Diffuse at bud and more at neck | |
| VPS34 | >50% R | Round 3, large 3 | WT | WT | |
| VAM3 | >50% R | Round 3 | WT | Most or all of the bud periphery | |
| VAC7 | >50% R | Large 2 | NT | WT | |
| END3 | >50% R | Small 3, round 2 | NT | More concentrated at the tip | |
| COS16 | >50% R | Rounds 2+ | NT | NT | |
| Weak phenotype | LUV1 | <50% R | Round 2 | WT | |
| CUP5 | <50% R | Round 3 | WT | ||
| VPS45 | <20% R, <20% U | Elongate 3, branch | NT | ||
| SEC22 | 15% R | Large 2 | WT | ||
| SNC2 | 50% R | Round 3 | WT | ||
| Actin-Cytoskeleton | |||||
| Strong phenotype | SLA1 | >50% R | Round 3 | WT | |
| RVS167 | >50% R | Round 2, clumpy 3 | WT | ||
| CAP1 | >50% R | Large 3, round 3 | WT | Diffuse | |
| YKE2 | >50% R | Round 2 | WT | ||
| MDM20 | >50% R | Round 3 | WT | ||
| RVS161 | >50% R | Round 3 | WT | ||
| Bud site selection and cell polarity proteins | |||||
| Strong phenotype | RSR1 | >50% R | Small 2, round 2 | Random | |
| BUD2 | >50% R | WT | Random | ||
| SPA2 | >50% R | Round 3 | WT | Diffuse | |
| BUD6 | >50% R | Round 3 | WT | Diffuse | |
| ROM2 | >50% R | Large 3, round 3 | WT | ||
| BEM4 | >50% R | Round 3 | R; U; B | WT | |
| BNI1 | >50% R | Round 3 | WT | ND | |
| Cell wall proteins | |||||
| Strong phenotype | CWH8 | >50% R | Round 3, clumpy 2 | Random | ND |
| FKS1 | >50% R | Small/round 3− | WT | WT | |
| GAS1 | >50% R | Round 3, clumpy 3 | WT | 17% of the cells | |
| Weak phenotype | SLG1 | <20% R | Elongate 2 | WT | |
| KRE6 | <50% R | Round 2, clumpy 2 | WT | ||
| CCW12 | 40% R | Round 2 | NT | ||
| Lipid metabolism | |||||
| Strong phenotype | GUP1 | >50% R | Round 2+, small 2+ | WT | |
| SUR4 | >50% R | Round 2 | B; U; R | ||
| Weak phenotype | FEN1 | 23% U, 7% R | WT | NT | |
| ERG4 | 30% R, 20% U | Round 3 | WT | ||
| ERG3 | 40% U | Round 2 | WT | ||
| Protein modification | |||||
| Strong phenotype | PMT2 | >50% R | Small 2, round 2 | WT | |
| MAP1 | >50% R | Round 3 | WT | ||
| OST3 | >50% R | Small 3, round 3 | WT | ||
| LAS21 | >50% R | Round 4 | WT | ||
| NAT3 | >50% R | Round 3 | WT | ||
| RAD6 | >50% R | Large 3 or large/round 3 | WT | ||
| Weak phenotype | MNN2 | 30% R | Small 3, round 3 | NT | |
| Transcriptional proteins | |||||
| Strong phenotype | CCR4 | >50% R | Round 3 | WT | Weak or ND |
| NOT5 | >50% R | Round 2, clumpy 2 | WT | Weak or ND | |
| POP2 | >50% R | Round 3 | NT | Weak or ND | |
| RLR1 | >50% R | Elongate 2 | NT | ||
| CTK1 | >50% R | Round 3 | NT | ||
| SSN6 | >50% R | Round 3, clumpy 3 | NT | Weak or ND | |
| TUP1 | >50% R | Round 3, clumpy 3 | WT | Weak or ND | |
| HCR1 | >50% R | Small 3 | NT | ||
| Weak phenotype | SIN4 | 50% U, 50% B | Clumpy 3+ | WT | |
| CTK3 | 20% R, few U | WT | WT | ||
| GCR3 | 43% U | WT | WT | ||
| RPB4 | 30% U, few R | Elongate 2 | NT | ||
| Nuclear proteins | |||||
| Strong phenotype | SPO7 | >50% R | Round 3, large 2 | WT | |
| NPL3 | >50% R | WT | WT | ||
| NSR1 | >50% R | Football 2 | WT | ||
| SFP1 | >50% R | Small 2 | WT | ||
| NEM1 | >50% R | Round 2 | WT | ||
| HMO1 | >50% R | NT | NT | ||
| RAI1 | >50% R | Round 2 | NT | ||
| LSM1 | >50% R | Small 3, round 3 | WT | ||
| LOC1 | >50% R | Round 3 | WT | ||
| Weak phenotype | TOP3 | 40% R | Large 2, elongate 2 | WT | |
| TRF4 | 20% U or R | Large 3 | WT | ||
| ZUO1 | <50% U | Round 2 | NT | ||
| Other proteins | |||||
| Strong phenotype | HEM14 | >50% R | Round 3 | WT | |
| ATP14 | >50% R | Small 2 | WT | ||
| PRS3 | >50% R | Round 3 | WT | ||
| RIB4 | >50% R | Round 3 | WT | ||
| Weak phenotype | YPS7 | 30% R | Small 3/round 3 | WT | |
| PRO1 | 50% R | Large 3, football 3 | WT | ||
| ILM1 | 50% R | Round 3 | NT | ||
| Unknown protein | |||||
| Strong phenotype | BUD14/YAR014c | >50% R | Elongate 3 | WT | |
| BUD15/YBL047c | >50% R | WT | WT | ||
| BUD16/YEL029c | >50% R | Large 2, round 3 | WT | ||
| BUD17/YNR027w | >50% R | Round 3 | NT | ||
| BUD18/YER044c | >50% R | Small/round 3, clumpy 2 | WT | ||
| YGR151ca | >50% R | Round 3 | R | ||
| BUD19/YJL188c | >50% R | Round 3 | WT | ||
| BUD20/YLR074c | >50% R | Small/round 3 | WT | ||
| BUD21/YOR078w | >50% R | WT | WT | ||
| BUD22/YMR014w | >50% R | Small 2 | NT | ||
| BUD23/YCR047c | >50% R | NT | NT | ||
| Weak phenotype | BUD24/YDR320c | 50% R | Small/round 2+ | WT | |
| BUD25/YER014c-a | <50% R | WT | WT | ||
| BUD26/YDR241w | 40% R | Small 2 | WT | ||
| BUD27/YFL023w | 40% R | Football 3 | WT | ||
| BUD28/YLR062c | <50% R, 15% U | Round 3 | WT | ||
| BUD29/YOL072w | <40% R | Round 2 | WT | ||
| BUD30/YDL151c | 45% R, 10% U | Round 2 | NT | ||
| BUD31/YCR063w | 50% R, few U | Large 3, round 2 | NT | ||
| BUD32/YGR262c | 50% R | Large 2 | NT |
WT, wild-type; ND, not detected; NT, not tested; R, random; U, unipolar; B, bipolar.
a
Overlaps with BUD1.