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. 2017 Aug 21;7:8956. doi: 10.1038/s41598-017-09165-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Frequency of cTfh cells and of Th1-, Th2- and Th17-like cTfh cells. The frequency of cTfh cells (A) and Th1-, Th2- and Th17-like cTfh cells (B–D) was measured in specimens from healthy controls and HIV-1 infected children. 22 healthy controls (HC) and 19 HIV-1 infected children were examined at baseline (BL) and 36 HC and 25 HIV-1 infected children after 1 month from last vaccination. A correlation between CD4+ T cell count and Th17-like cTfh cells was found at baseline in HIV-1 infected children (E). Unpaired and paired T test was used to calculate the differences between and within the groups. ns = not statistical significant, *p < 0.05, and **p < 0.01.