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. 2017 Aug 21;7:8956. doi: 10.1038/s41598-017-09165-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CXCL13 levels in plasma of healthy controls and HIV-1 infected children prior to vaccination and at 1 and 6 months post vaccination. CXCL13 was measured prior to vaccination and at 1 and 6 months post vaccination in specimens from healthy controls (n = 63, 62 and 53) and HIV-1 infected children (n = 49, 47 and 41) (A). ANOVA was used to calculate the differences between healthy controls and HIV-1 infected children at the 3 time points. Unpaired T test was also used to calculate the differences between healthy controls and HIV-1 at individual time points. The lines represent the median values in the figures. ****p < 0.0001. A correlation was found between the plasma levels of CXCL13 and anti-HBs abs at 6 months from the last vaccination in healthy controls (panel B: n = 53). A significant correlation was found in the group of healthy controls at 1 month post vaccination (C; n = 35) and HIV-1 infected children when comparing the levels of plasmatic CXCL13 expression and frequency of cTfh cells at baseline (D; n = 19). HIV = HIV-1 infected children; HC = healthy controls.