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The effect of spent culture medium on persister cell formation by H. volcanii. Percentage survival of H. volcanii following incubation for 1 h in the presence of its own spent culture medium or the spent culture media of other haloarchaea or halophilic bacteria, followed by challenge for 6 h with 0.005% H2O2. Cells suspended in fresh broth served as a negative control. Plotted values are the mean of triplicate measurements and error bars represent ± SD.
