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. 2017 Aug 21;8:956. doi: 10.3389/fimmu.2017.00956

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Copyright © 2017 Tian, Wong, Uger, Wisniewski and Chao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Amino acid sequence of the V21H4 antibody. (B) Description of conjugation strategy used to conjugate V21H4 to urease to generate V21H4-DOS47. Step 1 is the activation of the antibody using BM(PEG)₂. Step 2 conjugates the activated antibody to urease. (C) RP-HPLC chromatograms of V21H4 samples at different refolding time points. Blue line: sample at refolding time 0, immediately after the SP pooled fraction was mixed with refolding buffer. Red line: refolding time point 2 h after mixing. Green line: refolding sample 4 h after time 0 and 2 h after addition of 1.2 mM cystamine. Unfolded antibody elutes at 12.513 min and folded antibody elutes at 10.958 min.