Table 2.
List of identified peptides and activation sites of V21H4-(PEG)2-Cys.
| Tryptic peptide | Activation site | Calculated mass (Da) | MS/MS b/y possible | MS/MS b/y found | Intensity | Mass match error (ppm) | % of activation |
|---|---|---|---|---|---|---|---|
| T001 | 1,985.0364 | 38 | 34 | 25539260 | 1.2 | ||
| T001–002 | 2,730.3904 | 54 | 17 | 55292 | 0.6 | ||
| T002 | 763.3647 | 14 | 8 | 7457241 | −0.7 | ||
| T002* | C23-BM(PEG)2-Cys | 1,192.4852 | 14 | 5 | 169047 | 2.1 | 2.2 |
| T002–003 | 2,066.9456 | 36 | nd | nd | nd | ||
| T003 | 1,321.5913 | 20 | 18 | 29459300 | −0.5 | ||
| T003–004 | 1,802.8562 | 30 | nd | nd | nd | ||
| T004 | 499.2754 | 8 | 5 | 254649 | −2.6 | ||
| T004–005 | 784.4191 | 12 | 8 | 1083205 | −2.7 | ||
| T005 | 303.1543 | 2 | 1 | 69756 | −4 | ||
| T005–006 | 2,503.1868 | 42 | 27 | 4016949 | 3 | ||
| T006 | 2,218.043 | 38 | 35 | 10074250 | −0.4 | ||
| T006–007 | 2,431.1655 | 42 | 2 | 57264 | 4 | ||
| T007 | 231.1331 | 2 | 1 | 168759 | −4.3 | ||
| T007–008 | 835.4664 | 12 | 10 | 1210281 | −2.9 | ||
| T008–009 | 1,050.5458 | 16 | 6 | 92188 | −2.4 | ||
| T009 | 446.2125 | 6 | 5 | 247926 | −0.9 | ||
| T009–010 | 3,129.49 | 54 | nd | nd | nd | ||
| T010 | 2,701.2881 | 46 | 35 | 62124531 | 1.3 | ||
| T010* | C97-BM(PEG)2-Cys | 3,130.4087 | 46 | 7 | 334626 | 3.3 | 0.5 |
| T010–011 | 5,613.6455 | 98 | nd | nd | nd | ||
| T011 | 2,930.3682 | 50 | 37 | 18549570 | −1.4 | ||
| T011–012 | 3,749.6023 | 68 | nd | nd | nd | ||
| T012 | 837.2446 | 16 | 8 | 150911 | −2.1 | ||
| T012* | C136-BM(PEG)2-Cys | 1,266.3652 | 16 | 10 | 1885506 | −0.2 | 92.6 |
Thick boxes (also shaded blue) around sets of tryptic peptides indicate related groups of peptides used to calculate percentage of activation for each activation site.
nd, not detected.
*indicates the modified version of the peptide.