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. 2017 Aug 21;7:8874. doi: 10.1038/s41598-017-09262-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of proximal tubule formation in the pronephros by the loss of Prdx1 and its rescue by Prdx1* RNA co-injection. (A) prdx1 MOs (40 ng) were injected into both blastomeres of two-cell stage embryos. Embryos at the stage 33 were used for whole-mount in situ hybridization and immunohistochemistry. Proximal tubules in the pronephros were visualized by whole-mount in situ hybridization using smp30, xPteg, and pax2 probes and by immunohistochemistry using a 3G8 antibody. Expression of the proximal tubule-specific markers smp30 (B), xPteg (C), and pax2 (D) in prdx1 MO-injected embryos compared with the controls. Co-injection with prdx1* RNA rescued the decreased expression. The immunohistochemistry for 3G8 patterns were similar with whole-mount in situ hybridization (E). prdx1 morphants exihibited inhibition of proximal tubule formation only while intermediate, distal and connecting tubules were not affected by loss of prdx1.