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. 2017 Aug 21;7:8348. doi: 10.1038/s41598-017-08864-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Restriction digestion of phage 9 g DNA by Type II REases. (A) Restriction digestion of phage 9 g DNA by Type II REases with A/T only or A/T rich recognition sequences. REases were indicated on top of each lane as well as the number of guanine bases (G) in the recognition sequence. The exact number of dG⁺ bases in a particular restriction site is unknown. Refer to computer-generated restriction patterns in the Supplementary Material for reference. (B) Restriction digestion of phage 9 g DNA by Type II REases and analysis of restricted fragments on 1% agarose gels.