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. 2017 Aug 21;7:8883. doi: 10.1038/s41598-017-09118-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of isolated compounds on iNOS enzyme activities and NO production in LPS-stimulated BV2 microglia cells. (A) Assays of iNOS enzyme activities. BV2 cells were pre-treated with all compounds (50 μM) for 1 h, and stimulated with 0.5 μg/mL LPS for 24 h. The activities of iNOS enzyme were determined by using nitric oxide synthase activity assay kit (Abcam). The results were presented as mean ± SD (n = 3) and analyzed by one-way ANOVA with Dunnett’s test. *p < 0.05; **p < 0.01; ***p < 0.001. (Drug treatment plus LPS vs LPS). (B) Determination of NO levels in culture medium. After the drug treatment as previously described, the culture medium was collected for measurement of NO production by using Griess reagent kit for nitrite determination. Data were expressed as mean ± SD (n = 3) and analyzed by one-way ANOVA with Dunnett’s test. *p < 0.05; **p < 0.01; ***p < 0.001. (Drug plus LPS vs LPS).