Figure S1. The FRET effect caused by G-quadruplex folding.

Fluorescence intensities of FAM (donor) at 525 nm and Hex (acceptor) at 595 nm verse time during the G-quadruplex folding process in 100 mM NaCl buffer at room temperature (23°C). The two measurements were performed separately (i.e., not simultaneously), because we had to change the optical filters. The solid lines are double-exponential fits of the data, yielding A_fast = 0.205 ± 0.003, A_slow = 0.059 ± 0.002, k_fast = 1.63 ± 0.02 s⁻¹, and k_slow = 0.26 ± 0.01 s⁻¹ for FAM; and A_fast = −0.193 ± 0.003, A_slow = −0.060 ± 0.001, k_fast = 1.52 ± 0.02 s⁻¹, and k_slow = 0.22 ± 0.01 s⁻¹ for Hex.

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