Fig. 9.

lapsyn is widely required for branch morphogenesis of astrocyte-like glia in the CNS. Brp was used to visualize synaptic neuropils (red, a–c, g–i). Fas2 labeled the mushroom body lobes (d–f, red). a, d, g The fosmid lapsyn ^fTRG027706 reported lapsyn expression (green, arrows) in adult astrocyte-like glia extending into glomeruli within the antennal lobe (a), the neuropils of the central brain (c), and the VNC (g) in addition to other glia. b, e, h loco ^1.3D2 -Gal4 UAS-cd8GFP (green) labeled branches of astrocyte-like glia in all examined CNS areas. In mushroom body α and β lobes (d, e, f, outlined), lapsyn expression was weak, in line with the low density of astrocyte-like glial branches labeled with loco ^1.3D2 -Gal4 UAS-cd8GFP, limiting assessment of defects following lapsyn knockdown. Compared to controls b, e, h, the knockdown of lapsyn in astrocyte-like glia resulted in reduced branch morphogenesis (arrows) in the adult antennal lobe, the central brain and VNC (c, f, i). All panels show single optical sections. For genotypes and sample numbers, see Supplementary Table 1. Scale bars, 50 μm