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. 2017 Aug 21;7:8882. doi: 10.1038/s41598-017-09370-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Sequential process of surface modification. (b) Aβ antibody immobilization and BSA binding for blocking of nonspecific binding. (c) Aβ capture test using the quantitative analysis of the intensity of the fluorescent signal. (i) the fluorescent image of Aβ reaction on Aβ antibody functionalized surface. (ii) a negative control on the Aβ reaction with BSA functionalized surface. (iii) the graph of fluorescent intensity on Fig. 2a(i) and (ii). (d,e) The fluorescence images show Aβ plaque in the brain slices of wildtype (d) and transgenic (e) mouse.