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. 2017 Aug 21;7:8329. doi: 10.1038/s41598-017-08060-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Predation in Ca/HEPES showing live bacterial numbers following pre-exposure of predator and prey to human serum. B. bacteriovorus HD100 and K. pneumoniae were exposed to human serum for two hours (t = −2 to 0 h). Predator and prey were then resuspended in Ca/HEPES and combined together in a predation assay and changes in the K. pneumoniae prey population enumerated periodically (Kp + HD100). As a control, K. pneumoniae prey previously exposed to human serum was resuspended in Ca/HEPES and enumerated periodically in the absence of B. bacteriovorus HD100 predator (Kp + Ca/HEPES). Arrows indicate the time at which bacteria were transferred from serum to buffer and subjected to respective treatment). B. bacteriovorus previously exposed to whole serum was added to K. pneumoniae to give a predator concentration at t = 0 h of approximately 5 × 10⁸ pfu/ml.