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. 2017 Aug 21;7:8329. doi: 10.1038/s41598-017-08060-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Predation in whole human serum by HD100 mCherry fluorescent predator. Changes in the population of both K. pneumoniae prey (Kp + HD100 mCherry) and B. bacteriovorus HD100 mCherry predator (HD100 mCherry + Kp) were enumerated periodically during the predation assay. As a control, K. pneumoniae prey suspended in serum was enumerated periodically in the absence of B. bacteriovorus HD100 mCherry predator (Kp alone). (b) Microscopic analysis of B. bacteriovorus morphology in human serum with K. pneumoniae prey. MicrobeJ software was used to automatically detect mCherry tagged fluorescent B. bacteriovorus cells and measure the roundness (4 × area / (π × major axis ²) of each individual cell at each time point. A roundness cut off of 0.65 was selected to distinguish between cells with typical vibroid morphology (roundness score < 0.65) and those with an abnormal round morphology (roundness score ≥ 0.65). Number of cells analysed ranged between 1026 and 109 cells. Exact sample sizes for each time point are given in Supplementary material, Figure S4.