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. 2017 Aug 21;7:9018. doi: 10.1038/s41598-017-09432-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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aFGF and ChABC promote astrocyte and Schwann cell integration at the interface of PNG and caudal spinal cord. Representative confocal images of sagittal sections showing PNG- (left column) or PNG+aFGF+ChABC- (right column) treated spinal cords 40 weeks post-injury. The top row shows low magnification of GFAP staining (white). Note the extensive migration of GFAP+ astrocytes throughout the PNG (GFAP-negative area) only following PNG+aFGF+ChABC treatment. Scale bar, 500 µm. Bottom row shows high magnification of PNG p75+ Schwann cells (red) and spinal cord GFAP+ astrocytes (green). Note the migration and integration of Schwann cells and astrocytes, including overlapping spatial domains (yellow), only following PNG+aFGF+ChABC treatment. Scale bar, 100 µm.